CHANGES.md

 # Changes
 
+v1.1.22 (dev)
+-------------
+* Documentation fixes (#79)
+* Fix for index error when running `detect` command (#80)
+
 v1.1.21 (2018.11.24)
 --------------------
 * Added long options for paired adapter parameters.

Makefile

@@ -19,8 +19,7 @@ test:
 	$(TEST)
 
 docs:
-	make -C docs api
-	make -C docs html
+	make -C doc html
 
 readme:
 	pandoc --from=markdown --to=rst --output=README.rst README.md
@@ -58,7 +57,7 @@ release:
 	$(TEST)
 	python setup.py sdist bdist_wheel
 	# release
-	python setup.py sdist upload
+	python setup.py sdist upload -r pypi
 	git push origin --tags
 	$(github_release)
 	$(docker)

README.md

-[![Travis CI](https://travis-ci.org/jdidion/atropos.svg?branch=master)](https://travis-ci.org/jdidion/atropos])
+[![Travis CI](https://travis-ci.org/jdidion/atropos.svg?branch=1.1)](https://travis-ci.org/jdidion/atropos)
 [![PyPi](https://img.shields.io/pypi/v/atropos.svg)](https://pypi.python.org/pypi/atropos)
 [![DOI](https://zenodo.org/badge/61393086.svg)](https://zenodo.org/badge/latestdoi/61393086)
 
@@ -127,12 +127,15 @@ The citation for the original Cutadapt paper is:
 * Provide option for RNA-seq data that will trim polyA sequence.
 * Add formal config file support (#53)
 * Automate crash reporting using [sentry](https://github.com/getsentry/raven-python).
+* Look at [NGMerge] for improving read merging: https://github.com/harvardinformatics/NGmerge
+* Look at replacing pysam with [pybam](https://github.com/JohnLonginotto/pybam)
 
 ### 1.4
 
 * Currently, InsertAligner requires a single 3' adapter for each end. Adapter trimming will later be generalized so that A) the InsertAligner can handle multiple matched pairs of adapters and/or B) multiple different aligners can be used for different adapters.
 * Integrate with [AdapterBase](https://github.com/NCBI-Hackathons/OnlineAdapterDatabase) for improved matching of detected contaminants to known adapters, automated trimming of datasets with known adapters, and (opt-in) submission of adapter information for novel datasets.
 * Migrate to seqio (https://github.com/jdidion/seqio) for reading/writing sequence files.
+    * Also look at using HTSeq instead: https://github.com/simon-anders/htseq
 * General-purpose read filtering based on read ID: https://github.com/marcelm/cutadapt/issues/107.
 * Currently, SAM/BAM input files must be name sorted; add an option to 1) pre-sort reads inline using samtools or sambamba, or 2) cache each read in memory until its mate is found.
 
@@ -169,7 +172,9 @@ The citation for the original Cutadapt paper is:
 ### 2.0
 
 * Simplification of command line options, perhaps by further splitting functionality up into different sub-commands, but also by more intelligent choices for default option values based on context.
+
 * Consider adding additional report formats
+* Add fuzzy matching: https://github.com/Daniel-Liu-c0deb0t/Java-Fuzzy-Search
 * https://github.com/marcelm/cutadapt/issues/112
 * Performance enhancements using
     * http://numba.pydata.org/

atropos/_version.py

@@ -23,8 +23,8 @@ def get_keywords():
     # setup.py/versioneer.py will grep for the variable names, so they must
     # each be defined on a line of their own. _version.py will just call
     # get_keywords().
-    git_refnames = " (tag: 1.1.21)"
-    git_full = "1b7273b082551e80652a45df2736e5ffff8ce7f9"
+    git_refnames = " (tag: 1.1.22)"
+    git_full = "2b15c778f0ccf1d0fb753e4334fa6dc0048a9ee6"
     keywords = {"refnames": git_refnames, "full": git_full}
     return keywords
 

atropos/align/__init__.py

@@ -212,6 +212,12 @@ MatchInfo = namedtuple("MatchInfo", (
 # * http://www.combio.pl/alfree/tools/
 # * http://www.combio.pl/alfree
 # * http://bioinformatics.org.au/tools/decaf+py/
+# 12. https://github.com/sdu-hpcl/BGSA
+# 13. Train a NN to approximate the pairwise alignment distance
+# https://academic.oup.com/bioinformatics/advance-article-abstract/doi/10.1093/bioinformatics/bty887/5140215?redirectedFrom=fulltext
+# 14. Nucl2vec: https://github.com/prakharg24/Nucl2vec (local alignment only - could
+# it be adapted to semi-global?)
+# * Can re-implement in SpaCy? https://spacy.io/usage/vectors-similarity
 
 class InsertAligner(object):
     """Implementation of an insert matching algorithm.

atropos/commands/detect/__init__.py

@@ -8,7 +8,7 @@ from atropos.align import Aligner, SEMIGLOBAL
 from atropos.commands.base import (
     BaseCommandRunner, Pipeline, SingleEndPipelineMixin, PairedEndPipelineMixin)
 from atropos.util import (
-    reverse_complement, sequence_complexity, enumerate_range, run_interruptible)
+    reverse_complement, sequence_complexity, run_interruptible)
 
 # TODO: Test whether using rc=True in parse_known_contaminants is as fast
 # and as accurate as testing both the forward and reverse complement
@@ -88,6 +88,8 @@ class CommandRunner(BaseCommandRunner):
             logging.getLogger().debug(
                 "Detecting contaminants using the kmer-based algorithm")
             detector_class = KhmerDetector
+        else:
+            raise ValueError("Invalid value for 'detector': {}".format(detector))
 
         summary_args = dict(
             kmer_size=kmer_size, n_reads=n_reads,
@@ -113,6 +115,7 @@ class CommandRunner(BaseCommandRunner):
         self.summary.update(mode='serial', threads=1)
         return run_interruptible(detector, self, raise_on_error=True)
 
+
 class Match(object):
     """A contaminant match.
 
@@ -224,6 +227,7 @@ class Match(object):
                 known_seqs=self.known_seqs)
         return summary
 
+
 class ContaminantMatcher(object):
     """Matches a known contaminant against other sequences.
 
@@ -279,7 +283,8 @@ class ContaminantMatcher(object):
             match_frac1 = n_matches / self.n_kmers
         if len(kmers) > 0:
             match_frac2 = n_matches / len(kmers)
-        return (match_frac1, match_frac2, compare_seq)
+        return match_frac1, match_frac2, compare_seq
+
 
 def create_contaminant_matchers(contaminants, kmer_size):
     """Create :class:`ContaminantMatcher`s from sequences.
@@ -296,6 +301,7 @@ def create_contaminant_matchers(contaminants, kmer_size):
         for seq, names in contaminants.iter_sequences()
     ]
 
+
 class Detector(SingleEndPipelineMixin, Pipeline):
     """Base class for contaminant detectors.
 
@@ -304,7 +310,7 @@ class Detector(SingleEndPipelineMixin, Pipeline):
         n_reads: Number of reads to sample.
         overrep_cutoff: Degree of overrepresentation required for a kmer to be
             considered as a contaminant.
-        known_contaminant: :class:`ContaminantMatcher`s to match against.
+        known_contaminants: :class:`ContaminantMatcher`s to match against.
         past_end_bases: On Illumina, long runs of A (and sometimes other bases)
             can signify that the sequencer has read past the end of a fragment
             that is shorter than the read length + adapter length. Those
@@ -392,39 +398,39 @@ class Detector(SingleEndPipelineMixin, Pipeline):
         for match in matches:
             match.estimate_abundance(self._read_sequences)
 
-        def _filter(match):
-            if match.count < self.min_report_freq:
+        def _filter(_match):
+            if _match.count < self.min_report_freq:
                 logging.getLogger().debug(
                     "Filtering {} because frequency {} < {}".format(
-                        match.seq, match.count, self.min_report_freq))
+                        _match.seq, _match.count, self.min_report_freq))
                 return False
-            if min_len and len(match) < min_len:
+            if min_len and len(_match) < min_len:
                 logging.getLogger().debug(
                     "Filtering {} because it's too short (< {} bp)".format(
-                        match.seq, min_len))
+                        _match.seq, min_len))
                 print('too short')
                 return False
-            if min_complexity and match.seq_complexity < min_complexity:
+            if min_complexity and _match.seq_complexity < min_complexity:
                 logging.getLogger().debug(
                     "Filtering {} because its complexity {} < {}".format(
-                        match.seq, match.seq_complexity, min_complexity))
+                        _match.seq, _match.seq_complexity, min_complexity))
                 return False
-            if self.include == 'known' and not match.is_known:
+            if self.include == 'known' and not _match.is_known:
                 logging.getLogger().debug(
                     "Filtering {} because it's not known".format(
-                        match.seq))
+                        _match.seq))
                 return False
-            elif self.include == 'unknown' and match.is_known:
+            elif self.include == 'unknown' and _match.is_known:
                 logging.getLogger().debug(
                     "Filtering {} because it's known".format(
-                        match.seq))
+                        _match.seq))
                 return False
             if (
-                    min_match_frac and match.is_known and
-                    match.match_frac < min_match_frac):
+                    min_match_frac and _match.is_known and
+                    _match.match_frac < min_match_frac):
                 logging.getLogger().debug(
                     "Filtering {} because its match_frac {} < {}".format(
-                        match.seq, match.match_frac, min_match_frac))
+                        _match.seq, _match.match_frac, min_match_frac))
                 return False
             return True
 
@@ -451,6 +457,7 @@ class Detector(SingleEndPipelineMixin, Pipeline):
             for match in self.matches(**kwargs)
         ],)
 
+
 class PairedDetector(PairedEndPipelineMixin, Pipeline):
     """Detector for paired-end reads.
     """
@@ -484,6 +491,7 @@ class PairedDetector(PairedEndPipelineMixin, Pipeline):
             for match in self.read2_detector.matches(**kwargs)
         ])
 
+
 class KnownContaminantDetector(Detector):
     """Test known contaminants against reads. This has linear complexity and is
     more specific than the khmer matcher, but less specific than the heuristic
@@ -540,6 +548,7 @@ class KnownContaminantDetector(Detector):
             )
         ]
 
+
 class HeuristicDetector(Detector):
     """Use a heuristic iterative algorithm to arrive at likely contaminants.
     This is the most accurate algorithm overall, but it has quadratic complexity
@@ -557,11 +566,11 @@ class HeuristicDetector(Detector):
         return 0.1 * self.n_reads
 
     def _get_contaminants(self):
-        def _min_count(kmer_size):
+        def _min_count(_kmer_size):
             return math.ceil(self.n_reads * max(
                 self.min_frequency,
-                (self._read_length - kmer_size + 1) * self.overrep_cutoff /
-                float(4**kmer_size)))
+                (self._read_length - _kmer_size + 1) * self.overrep_cutoff /
+                float(4 ** _kmer_size)))
 
         kmer_size = self.kmer_size
         kmers = defaultdict(lambda: [0, set()])
@@ -613,8 +622,7 @@ class HeuristicDetector(Detector):
 
         # Now merge overlapping sequences by length and frequency to eliminate
         # redundancy in the set of candidate kmers.
-        results.sort(key=lambda i: len(i[0]) * math.log(i[1]), reverse=True)
-        cur = results[0]
+        results.sort(key=lambda r: len(r[0]) * math.log(r[1]), reverse=True)
         merged = []
         unmerged = []
         while len(results) > 1:
@@ -643,7 +651,7 @@ class HeuristicDetector(Detector):
         # appear in full very infrequently
 
         # Re-sort by frequency
-        results.sort(key=lambda i: i[1], reverse=True)
+        results.sort(key=lambda r: r[1], reverse=True)
         # Keep anything that's within 50% of the top hit
         # TODO: make this user-configurable?
         min_count = int(results[0][1] * 0.5)
@@ -660,28 +668,33 @@ class HeuristicDetector(Detector):
             known = {}
             unknown = []
 
-            def find_best_match(seq, best_matches, best_match_frac):
+            def find_best_match(_seq, _best_matches, _best_match_frac):
                 """Find best contaminant matches to `seq`.
                 """
-                seqrc = reverse_complement(seq)
-                for contam in contaminants:
-                    match_frac1, match_frac2, compare_seq = contam.match(
-                        seq, seqrc)
-                    if match_frac1 < best_match_frac[0]:
+                seqrc = reverse_complement(_seq)
+
+                for _contam in contaminants:
+                    match_frac1, match_frac2, compare_seq = _contam.match(
+                        _seq, seqrc)
+                    if match_frac1 < _best_match_frac[0]:
                         continue
                     if (
-                            contam.seq in compare_seq or
+                            _contam.seq in compare_seq or
                             align(
-                                compare_seq, contam.seq,
+                                compare_seq, _contam.seq,
                                 self.min_contaminant_match_frac)):
-                        if (match_frac1 > best_match_frac[0] or (
-                                match_frac1 == best_match_frac[0] and
-                                match_frac2 > best_match_frac[1])):
-                            best_matches = {}
-                            best_match_frac = (match_frac1, match_frac2)
-                        best_matches[contam] = (
+                        if (
+                            match_frac1 > _best_match_frac[0] or (
+                                match_frac1 == _best_match_frac[0] and
+                                match_frac2 > _best_match_frac[1]
+                            )
+                        ):
+                            _best_matches = {}
+                            _best_match_frac = (match_frac1, match_frac2)
+                        _best_matches[_contam] = (
                             match, (match_frac1, match_frac2))
-                return (best_matches, best_match_frac)
+
+                return _best_matches, _best_match_frac
 
             for match in matches:
                 best_matches, best_match_frac = find_best_match(
@@ -692,9 +705,9 @@ class HeuristicDetector(Detector):
                         match.longest_match[0], best_matches, best_match_frac)
 
                 if best_matches:
-                    for contam, match in best_matches.items():
-                        if contam not in known or match[1] > known[contam][1]:
-                            known[contam] = match
+                    for contam, _match in best_matches.items():
+                        if contam not in known or _match[1] > known[contam][1]:
+                            known[contam] = _match
                 else:
                     unknown.append(match)
 
@@ -720,7 +733,7 @@ class HeuristicDetector(Detector):
                         match.set_contaminant(contam, *match_frac)
                     else:
                         names = set(contam.names)
-                        seqs = set((contam.seq,))
+                        seqs = {(contam.seq,)}
                         for other_contam in equiv:
                             names.update(other_contam[0].names)
                             seqs.add(other_contam[0].seq)
@@ -731,6 +744,7 @@ class HeuristicDetector(Detector):
 
         return matches
 
+
 class KhmerDetector(Detector):
     """Identify contaminants based on kmer frequency using a fast kmer counting
     approach (as implemented in the khmer library). This approach is fast but
@@ -770,12 +784,12 @@ class KhmerDetector(Detector):
             matches = []
             seen = set()
 
-            def match(kmer):
+            def match(_kmer):
                 """Returns the frequency of `kmer` in `candidates`.
                 """
-                freq = candidates.get(kmer, 0)
+                freq = candidates.get(_kmer, 0)
                 if freq > 0:
-                    seen.add(kmer)
+                    seen.add(_kmer)
                 return freq
 
             for seq, names in self.known_contaminants.iter_sequences():
@@ -817,11 +831,13 @@ class KhmerDetector(Detector):
 
         return matches
 
+
 def align(seq1, seq2, min_overlap_frac=0.9):
     """Align two sequences.
 
     Args:
-        seq1, seq2: The sequences to align.
+        seq1: The second sequence to align.
+        seq2: The first sequence to align.
         min_overlap_frac: Minimum fraction of overlapping bases required for a
             match.
 

debian/changelog

+atropos (1.1.22+dfsg-1) unstable; urgency=medium
+
+  * Team upload.
+  * New upstream version
+  * debhelper-compat 12
+
+ -- Steffen Moeller <moeller@debian.org>  Thu, 21 Nov 2019 16:24:36 +0100
+
 atropos (1.1.21+dfsg-1) unstable; urgency=medium
 
   * Initial release (Closes: #926944)

debian/compat

-12

debian/control

@@ -3,7 +3,7 @@ Maintainer: Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.
 Uploaders: Andreas Tille <tille@debian.org>
 Section: science
 Priority: optional
-Build-Depends: debhelper (>= 12~),
+Build-Depends: debhelper-compat (= 12),
                dh-python,
                cython3,
                python3-dev,
@@ -11,7 +11,7 @@ Build-Depends: debhelper (>= 12~),
                python3-pytest <!nocheck>,
                python3-jinja2 <!nocheck>,
                python3-pysam <!nocheck>
-Standards-Version: 4.3.0
+Standards-Version: 4.4.1
 Vcs-Browser: https://salsa.debian.org/med-team/atropos
 Vcs-Git: https://salsa.debian.org/med-team/atropos.git
 Homepage: https://github.com/jdidion/atropos

debian/copyright

@@ -2,6 +2,17 @@ Format: https://www.debian.org/doc/packaging-manuals/copyright-format/1.0/
 Upstream-Name: atropos
 Source: https://github.com/jdidion/atropos/releases
 Files-Excluded: *.tar.gz
+ .pytest_cache/README.md
+ .pytest_cache/v/cache/nodeids
+ .pytest_cache/v/cache/stepwise
+ atropos.egg-info/PKG-INFO
+ atropos.egg-info/SOURCES.txt
+ atropos.egg-info/dependency_links.txt
+ atropos.egg-info/requires.txt
+ atropos.egg-info/top_level.txt
+ atropos/align/_align.c
+ atropos/commands/trim/_qualtrim.c
+ atropos/io/_seqio.c
 
 Files: *
 Copyright: 2010-2016 Marcel Martin <marcel.martin@scilifelab.se>

debian/rules

@@ -19,3 +19,10 @@ override_dh_install:
 	find debian -type d -name __pycache__ | xargs rm -rf
 	find debian -type d -name tests       | xargs rm -rf
 	find debian -type f -name .adapters -delete
+
+override_dh_auto_clean:
+	dh_auto_clean
+	rm -rf  .pytest_cache
+	rm -rf atropos.egg-info
+	rm -f atropos/align/_align.c atropos/commands/trim/_qualtrim.c atropos/io/_seqio.c
+

doc/conf.py

@@ -46,7 +46,7 @@ master_doc = 'index'
 
 # General information about the project.
 project = u'atropos'
-copyright = u'Original Cutadapt code: 2010-2014, Marcel Martin; Atropos modifications and improvements: 2015-2016, John P Didion'
+copyright = u'Original Cutadapt code: 2010-2014, Marcel Martin; Atropos modifications and improvements: 2015-2018, John P Didion'
 
 # The version info for the project you're documenting, acts as replacement for
 # |version| and |release|, also used in various other places throughout the
@@ -208,8 +208,7 @@ latex_elements = {
 # (source start file, target name, title,
 #  author, documentclass [howto, manual, or own class]).
 latex_documents = [
-  ('index', 'atropos.tex', u'atropos Documentation',
-   u'Marcel Martin', 'manual'),
+  ('index', 'atropos.tex', u'Atropos Documentation', u'John Didion', 'manual'),
 ]
 
 # The name of an image file (relative to this directory) to place at the top of
@@ -238,8 +237,7 @@ latex_documents = [
 # One entry per manual page. List of tuples
 # (source start file, name, description, authors, manual section).
 man_pages = [
-    ('index', 'atropos', u'atropos Documentation',
-     [u'Marcel Martin'], 1)
+    ('index', 'atropos', u'Atropos Documentation', [u'John Didion'], 1)
 ]
 
 # If true, show URL addresses after external links.
@@ -252,9 +250,8 @@ man_pages = [
 # (source start file, target name, title, author,
 #  dir menu entry, description, category)
 texinfo_documents = [
-  ('index', 'atropos', u'atropos Documentation',
-   u'Marcel Martin', 'atropos', 'One line description of project.',
-   'Miscellaneous'),
+  ('index', 'atropos', u'atropos Documentation', u'John Didion', 'atropos',
+   'General purpose NGS read manipulation tool.', 'Read manipulation'),
 ]
 
 # Documents to append as an appendix to all manuals.

doc/guide.rst

@@ -657,10 +657,10 @@ been removed. For example, if the following sequence is in reads.fastq::
     ACGTACGTACGTADAP
 
 The following command will first trim the ``ADAP`` part of the adapter
-off the end. Then, since only 4 bases were trimmed, the ``-i 5`` option
+off the end. Then, since only 4 bases were trimmed, the ``-i -5`` option
 will cause a 5th base to be removed.
 
-    atropos -A ADAPTER -i 5 -o trimmed.fastq -se reads.fastq
+    atropos -A ADAPTER -i -5 -o trimmed.fastq -se reads.fastq
 
 .. _quality-trimming:
 

doc/index.rst

-.. include:: ./readme.rst
-
 =================
 Table of contents
 =================

setup.py

@@ -146,7 +146,7 @@ setup(
     url="https://atropos.readthedocs.org/",
     description="trim adapters from high-throughput sequencing reads",
     long_description=long_description,
-    log_description_content_type="text/markdown",
+    long_description_content_type="text/markdown",
     license="Original Cutadapt code is under MIT license; improvements and additions are in the Public Domain",
     ext_modules=extensions,
     packages=find_packages(),