Loading ChangeLog.txt +12 −1 Original line number Diff line number Diff line 2018-12-26 Martin C. Frith <Martin C. Frith> * src/tantan.cc: Make tantan repeat-finding faster [44199bff9cef] [tip] * doc/Makefile, doc/last-parallel.txt, doc/last-split.txt, doc/last- tutorial.txt: Update the documents a bit [6727ae8b7a12] 2018-12-10 Martin C. Frith <Martin C. Frith> * doc/last-dotplot.txt, scripts/last-dotplot: Add last-dotplot --maxseqs option [ed0fb9b1eb40] [tip] [ed0fb9b1eb40] * src/split/last-split.cc: Make last-split -n keep E-values Loading doc/Makefile +1 −0 Original line number Diff line number Diff line Loading @@ -15,6 +15,7 @@ ${DOCS}: last-doc.css Makefile # Ugh! Is there a better way? RST_CSS = `locate html4css1.css | tail -n1` #RST_CSS = html4css1.css RSTFLAGS = --initial-header-level=2 --no-compact-lists \ --no-compact-field-lists --option-limit=0 --no-doc-info Loading doc/last-parallel.html +2 −2 Original line number Diff line number Diff line Loading @@ -364,11 +364,11 @@ parallel-fasta "lastal mydb" < queries.fa > myalns.maf </pre> <p>Instead of this:</p> <pre class="literal-block"> lastal -Q1 -D100 db q.fastq | last-split > out.maf lastal -Q1 db q.fastq | last-split > out.maf </pre> <p>try this:</p> <pre class="literal-block"> parallel-fastq "lastal -Q1 -D100 db | last-split" < q.fastq > out.maf parallel-fastq "lastal -Q1 db | last-split" < q.fastq > out.maf </pre> <p>Instead of this:</p> <pre class="literal-block"> Loading doc/last-parallel.txt +2 −2 Original line number Diff line number Diff line Loading @@ -53,11 +53,11 @@ try this:: Instead of this:: lastal -Q1 -D100 db q.fastq | last-split > out.maf lastal -Q1 db q.fastq | last-split > out.maf try this:: parallel-fastq "lastal -Q1 -D100 db | last-split" < q.fastq > out.maf parallel-fastq "lastal -Q1 db | last-split" < q.fastq > out.maf Instead of this:: Loading doc/last-split.html +3 −3 Original line number Diff line number Diff line Loading @@ -334,7 +334,7 @@ format), and the genome is in "genome.fasta" (in fasta format). We can do the alignment like this:</p> <pre class="literal-block"> lastdb -uNEAR -R01 db genome.fasta lastal -Q1 -D100 db q.fastq | last-split > out.maf lastal -Q1 db q.fastq | last-split > out.maf </pre> </div> <div class="section" id="spliced-alignment-of-rna-reads-to-a-genome"> Loading Loading @@ -397,7 +397,7 @@ matches.</p> <h2>Going faster by parallelization</h2> <p>For example, split alignment of DNA reads to a genome:</p> <pre class="literal-block"> parallel-fastq "lastal -Q1 -D100 db | last-split" < q.fastq > out.maf parallel-fastq "lastal -Q1 db | last-split" < q.fastq > out.maf </pre> <p>This requires GNU parallel to be installed (<a class="reference external" href="http://www.gnu.org/software/parallel/">http://www.gnu.org/software/parallel/</a>).</p> Loading Loading @@ -558,7 +558,7 @@ middle of the query, we can do "spliced" alignment without considering splice signals or favouring cis-splices:</p> <pre class="literal-block"> lastdb -uNEAR -R01 db genome.fasta lastal -Q1 -D100 db q.fastq | last-split -c0 > out.maf lastal -Q1 db q.fastq | last-split -c0 > out.maf </pre> </div> </div> Loading Loading
ChangeLog.txt +12 −1 Original line number Diff line number Diff line 2018-12-26 Martin C. Frith <Martin C. Frith> * src/tantan.cc: Make tantan repeat-finding faster [44199bff9cef] [tip] * doc/Makefile, doc/last-parallel.txt, doc/last-split.txt, doc/last- tutorial.txt: Update the documents a bit [6727ae8b7a12] 2018-12-10 Martin C. Frith <Martin C. Frith> * doc/last-dotplot.txt, scripts/last-dotplot: Add last-dotplot --maxseqs option [ed0fb9b1eb40] [tip] [ed0fb9b1eb40] * src/split/last-split.cc: Make last-split -n keep E-values Loading
doc/Makefile +1 −0 Original line number Diff line number Diff line Loading @@ -15,6 +15,7 @@ ${DOCS}: last-doc.css Makefile # Ugh! Is there a better way? RST_CSS = `locate html4css1.css | tail -n1` #RST_CSS = html4css1.css RSTFLAGS = --initial-header-level=2 --no-compact-lists \ --no-compact-field-lists --option-limit=0 --no-doc-info Loading
doc/last-parallel.html +2 −2 Original line number Diff line number Diff line Loading @@ -364,11 +364,11 @@ parallel-fasta "lastal mydb" < queries.fa > myalns.maf </pre> <p>Instead of this:</p> <pre class="literal-block"> lastal -Q1 -D100 db q.fastq | last-split > out.maf lastal -Q1 db q.fastq | last-split > out.maf </pre> <p>try this:</p> <pre class="literal-block"> parallel-fastq "lastal -Q1 -D100 db | last-split" < q.fastq > out.maf parallel-fastq "lastal -Q1 db | last-split" < q.fastq > out.maf </pre> <p>Instead of this:</p> <pre class="literal-block"> Loading
doc/last-parallel.txt +2 −2 Original line number Diff line number Diff line Loading @@ -53,11 +53,11 @@ try this:: Instead of this:: lastal -Q1 -D100 db q.fastq | last-split > out.maf lastal -Q1 db q.fastq | last-split > out.maf try this:: parallel-fastq "lastal -Q1 -D100 db | last-split" < q.fastq > out.maf parallel-fastq "lastal -Q1 db | last-split" < q.fastq > out.maf Instead of this:: Loading
doc/last-split.html +3 −3 Original line number Diff line number Diff line Loading @@ -334,7 +334,7 @@ format), and the genome is in "genome.fasta" (in fasta format). We can do the alignment like this:</p> <pre class="literal-block"> lastdb -uNEAR -R01 db genome.fasta lastal -Q1 -D100 db q.fastq | last-split > out.maf lastal -Q1 db q.fastq | last-split > out.maf </pre> </div> <div class="section" id="spliced-alignment-of-rna-reads-to-a-genome"> Loading Loading @@ -397,7 +397,7 @@ matches.</p> <h2>Going faster by parallelization</h2> <p>For example, split alignment of DNA reads to a genome:</p> <pre class="literal-block"> parallel-fastq "lastal -Q1 -D100 db | last-split" < q.fastq > out.maf parallel-fastq "lastal -Q1 db | last-split" < q.fastq > out.maf </pre> <p>This requires GNU parallel to be installed (<a class="reference external" href="http://www.gnu.org/software/parallel/">http://www.gnu.org/software/parallel/</a>).</p> Loading Loading @@ -558,7 +558,7 @@ middle of the query, we can do "spliced" alignment without considering splice signals or favouring cis-splices:</p> <pre class="literal-block"> lastdb -uNEAR -R01 db genome.fasta lastal -Q1 -D100 db q.fastq | last-split -c0 > out.maf lastal -Q1 db q.fastq | last-split -c0 > out.maf </pre> </div> </div> Loading