The $k$-mer length is the length of the nodes in the de Bruijn graph. It strongly depends on the input dataset. For proper assembly, we recommend that you use the Minia-pipeline that runs Minia multiple times, with an iterative multi-k algorithm. That way, you won't need to choose k. If you insist on running with a single k value, the KmerGenie software can automatically find the best $k$ for your dataset.
The $k$-mer length is the length of the nodes in the de Bruijn graph. It strongly depends on the input dataset. For proper assembly, we recommend that you use the Minia-pipeline (\url{https://github.com/GATB/gatb-minia-pipeline}) that runs Minia multiple times, using an iterative multi-k algorithm (similar to IDBA, Megahit). That way, you won't need to choose k. If you insist on running with a single k value, the KmerGenie software can automatically find the best $k$ for your dataset.
\vitem+abundance-min+
The \verb+abundance-min+ is a hard cut-off to remove likely erroneous, low-abundance $k$-mers. In Minia 3, it is recommended to set it to 2 unless you have a good reason not to. Minia 3 also implements other ways to remove erroneous k-mers, using a more adequate relative cut-off, in the graph simplifications step.
@@ -113,11 +114,17 @@ Minia can direclty read files compressed with gzip. Compressed files should end
\section{Output}
The output of Minia is a set of contigs in the FASTA format, in the file \verb+[prefix].contigs.fa+.
\subsection{Fasta}
\subsection*{Creating unitigs}
The main output of Minia is a set of contigs in the FASTA format, in the file \verb+[prefix].contigs.fa+.
Minia will also output unitigs in the FASTA format. Those are non-branching paths in the de Bruijn graph prior to any graph simplification. File: \verb+[prefix].unitigs.fa+.
\subsection{Assembly graph}
The contigs FASTA file can be converted to GFA using \href{https://github.com/GATB/bcalm/blob/master/scripts/convertToGFA.py}{BCALM's convertToGFA.py script}.
\subsection*{Unitigs}
Minia will also output unitigs, in the FASTA format. They correspond to non-branching paths in the de Bruijn graph prior to any graph simplification. File: \verb+[prefix].unitigs.fa+.