.gitignore

@@ -4,6 +4,7 @@ doc/_build
 *~
 *.pyc
 *.egg-info
+.eggs/
 nosetests.xml
 coverage.xml
 .coverage

.pylintrc

 [MASTER]
-extension-pkg-whitelist=numpy,xlml.etree,pysam.samtools,h5py.h5s
+extension-pkg-whitelist=numpy,xlml.etree,pysam.samtools
 
 [TYPECHECK]
 
@@ -7,7 +7,7 @@ extension-pkg-whitelist=numpy,xlml.etree,pysam.samtools,h5py.h5s
 # (useful for modules/projects where namespaces are manipulated during runtime
 # and thus existing member attributes cannot be deduced by static analysis. It
 # supports qualified module names, as well as Unix pattern matching.
-ignored-modules=numpy,lxml.etree,pysam.samtools,h5py.h5s
+ignored-modules=numpy,lxml.etree,pysam.samtools
 
 # List of classes names for which member attributes should not be checked
 # (useful for classes with attributes dynamically set). This supports can work

CHANGELOG.org

+* Version 2.0
+  - Python 3.7 port, removed support for Python 2.x
+
 * Version 1.4.0
   - Support for Vcf4Records and merging VCF files
 

Makefile

@@ -15,7 +15,11 @@ install:
 	@pip install ./
 
 pylint:
-	pylint --errors-only --ignore=pyxb pbcore/
+	pylint --errors-only --ignore=pyxb --enable=C0411,W0702,W0401,W0611 pbcore/
+
+autopep8:
+	find pbcore -name "*.py" | xargs autopep8 -i
+	find tests -name "*.py" | xargs autopep8 -i
 
 clean: doc-clean
 	rm -rf build/;\
@@ -33,8 +37,7 @@ doctest:
 	cd doc && make doctest
 
 unit-test:
-	#nosetests --with-coverage --cover-xml-file=coverage.xml --cover-package=pbcore --cover-xml --with-xunit -v tests
-	pytest -v -n auto --dist=loadscope --durations=20 --junitxml=nosetests.xml --cov=./pbcore --cov-report=xml:coverage.xml tests/test_*.py
+	python setup.py test
 	sed -i -e 's@filename="@filename="./@g' coverage.xml
 
 test: doctest unit-test

bamboo_build.sh

 #!/bin/bash
 type module >& /dev/null || . /mnt/software/Modules/current/init/bash
-module load python/2
+module load python/3
+module load htslib  # since pysam was built against this
 set -ex
 nproc
 
@@ -20,11 +21,8 @@ fi
 
 rm -rf build
 mkdir -p build/bin build/lib build/include build/share
-$PIP install --user --no-index --find-link $WHEELHOUSE --no-compile -e .[test]
-$PIP install --user --no-index --find-link $WHEELHOUSE pbtestdata
-$PIP install --user --no-index --find-link $WHEELHOUSE pytest-xdist
-$PIP install --user --no-index --find-link $WHEELHOUSE pytest-cov
-#$PIP install --user --no-index --find-link $WHEELHOUSE pytest-parallel # not sure why this fails
+$PIP install --user --no-index --find-link $WHEELHOUSE --no-compile -e '.[test]'
+
 pytest --trace-config --collect-only
 
 set +e

bamboo_wheel.sh

@@ -23,18 +23,9 @@ export WHEELHOUSE=./wheelhouse
 # Give everybody read/write access.
 umask 0000
 
-
-module load python/2-UCS2
-make wheel
-
-# For now, we have only "any" wheels, so we do not need to build again.
-
-module unload python
-
-module load python/2-UCS4
+module load python/3
 make wheel
 
-
 # http://bamboo.pacificbiosciences.com:8085/build/admin/edit/defaultBuildArtifact.action?buildKey=SAT-TAGDEPS-JOB1
 # For old artifact config:
 #mkdir -p ./artifacts/gcc-6.4.0/wheelhouse

bin/updateXSDs.py

 #!/usr/bin/env python
 
-from __future__ import print_function
 import argparse
 import tempfile
 import shutil

debian/changelog

-python-pbcore (1.7.1+dfsg-1) UNRELEASED; urgency=medium
+python-pbcore (1.7.1+git20191121.7947eb7+dfsg-1) UNRELEASED; urgency=medium
 
   * Team upload.
-  * New upstream version
+  * New upstream version Git checkout
   * debhelper-compat 12
   * Drop Python2 support and switch to Python3
-    --> not supported upstream see https://github.com/PacificBiosciences/pbcore/issues/121
-  * Standards-Version: 4.4.0
+    --> discussed at https://github.com/PacificBiosciences/pbcore/issues/121
+  * Standards-Version: 4.4.1
+  * (Build-)Depends: python3-biopython (>= 1.74)
+  TODO: #944242 (Needs biopython 1.74)
 
  -- Andreas Tille <tille@debian.org>  Wed, 04 Sep 2019 10:29:56 +0200
 

debian/control

@@ -13,9 +13,11 @@ Build-Depends: debhelper-compat (= 12),
                python3-nose <!nocheck>,
                python3-pyxb <!nocheck>,
                python3-uritools <!nocheck>,
+               python3-pytest-runner <!nocheck>,
+               python3-biopython (>= 1.74) <!nocheck>,
                pylint3,
                python3-coverage
-Standards-Version: 4.4.0
+Standards-Version: 4.4.1
 Vcs-Browser: https://salsa.debian.org/med-team/python-pbcore
 Vcs-Git: https://salsa.debian.org/med-team/python-pbcore.git
 Homepage: https://github.com/PacificBiosciences/pbcore
@@ -24,7 +26,8 @@ Package: python3-pbcore
 Architecture: all
 Depends: ${misc:Depends},
          ${python3:Depends},
-         python3-pkg-resources
+         python3-pkg-resources,
+         python3-biopython (>= 1.74)
 Recommends: python3-h5py
 Suggests: python-pbcore-doc (= ${source:Version})
 Description: Python 3 library for processing PacBio data files

debian/patches/doc-theme.patch

@@ -7,9 +7,9 @@ Forwarded: no
 Last-Update: 2015-06-21
 ---
 This patch header follows DEP-3: http://dep.debian.net/deps/dep3/
---- python-pbcore.orig/doc/conf.py
-+++ python-pbcore/doc/conf.py
-@@ -99,7 +99,7 @@
+--- a/doc/conf.py
++++ b/doc/conf.py
+@@ -102,7 +102,7 @@ pygments_style = 'sphinx'
  
  # The theme to use for HTML and HTML Help pages.  See the documentation for
  # a list of builtin themes.
@@ -18,7 +18,7 @@ This patch header follows DEP-3: http://dep.debian.net/deps/dep3/
  
  # Theme options are theme-specific and customize the look and feel of a theme
  # further.  For a list of options available for each theme, see the
-@@ -108,7 +108,6 @@
+@@ -111,7 +111,6 @@ html_theme = 'pacbio-theme'
  
  # Add any paths that contain custom themes here, relative to this directory.
  #html_theme_path = []

debian/patches/enable-build-time-testing.patch

@@ -7,9 +7,12 @@ Forwarded: not-needed
 Last-Update: 2015-08-06
 --- a/setup.cfg
 +++ b/setup.cfg
-@@ -1,2 +1,5 @@
+@@ -1,5 +1,8 @@
  [metadata]
  description-file = README.md
 +[nosetests]
 +verbosity=2
 +where=tests
+ 
+ [aliases]
+ test = pytest

debian/patches/series

 doc-theme.patch
 enable-build-time-testing.patch
-ignore-pyxb.patch
-exclude_test_missing_data.patch
+# ignore-pyxb.patch
+# exclude_test_missing_data.patch

debian/watch

 version=4
 
-opts="repacksuffix=+dfsg,dversionmangle=s/\+dfsg//g,repack,compression=xz" \
-   https://github.com/PacificBiosciences/pbcore/releases .*/archive/v?@ANY_VERSION@@ARCHIVE_EXT@
+#opts="repacksuffix=+dfsg,dversionmangle=auto,repack,compression=xz" \
+#   https://github.com/PacificBiosciences/pbcore/releases .*/archive/v?@ANY_VERSION@@ARCHIVE_EXT@
 
 # Upstream is not tagging releases regularly - thus we use mode=git
-#opts="mode=git,pretty=1.5.5+git%cd.%h,repacksuffix=+dfsg,dversionmangle=s/\+dfsg//g,repack,compression=xz" \
-#  https://github.com/PacificBiosciences/pbcore.git HEAD
+# Upstream is now actually tagging releases - but there is no official Python3 release yet
+opts="mode=git,pretty=1.7.1+git%cd.%h,repacksuffix=+dfsg,dversionmangle=auto,repack,compression=xz" \
+  https://github.com/PacificBiosciences/pbcore.git HEAD

doc/conf.py

@@ -11,10 +11,13 @@
 # All configuration values have a default; values that are commented out
 # serve to show the default.
 
+import pkg_resources
 import sys, os
 
-# don't forget to update setup.py and pbcore/__init__.py too
-__VERSION__ = '1.7.1'
+try:
+    __VERSION__ = pkg_resources.get_distribution('pbcore').version
+except Exception:
+    __VERSION__ = 'unknown'
 
 
 # If extensions (or modules to document with autodoc) are in another directory,

doc/pbcore.io.dataset.rst

@@ -301,6 +301,7 @@ do them using the API, rather than the CLI.
     # open:
     dsets = [AlignmentSet(fn) for fn in filename_list]
     # merge with + operator:
+    from functools import reduce
     dset = reduce(lambda x, y: x + y, dsets)
 
     # OR:

doc/pbcore.io.rst

@@ -4,19 +4,15 @@ pbcore.io
 The ``pbcore.io`` package provides a number of lightweight interfaces
 to PacBio data files and other standard bioinformatics file formats.
 Preferred usage is to import classes directly from the ``pbcore.io``
-package, e.g.::
-
-    >>> from pbcore.io import CmpH5Reader
+package.
 
 The classes within ``pbcore.io`` adhere to a few conventions, in order
 to provide a uniform API:
 
   - Each data file type is thought of as a container of a `Record`
     type; all `Reader` classes support streaming access by iterating on the
-    reader object, and
-    `CmpH5Reader`, `BasH5Reader` and `IndexedBarReader` additionally 
-    provide random-access
-    to alignments/reads.
+    reader object, and `IndexedBarReader` additionally provides
+    random-access to alignments/reads.
 
     For example::
 
@@ -34,84 +30,20 @@ to provide a uniform API:
         for r in tqdm(f):
             process(r)
 
-
   - The constructor argument needed to instantiate `Reader` and
     `Writer` objects can be either a filename (which can be suffixed
-    by ".gz" for all but the h5 file types) or an open file handle.
+    by ".gz" for all file types) or an open file handle.
     The reader/writer classes will do what you would expect.
 
 
-  - The reader/writer classes all support the context manager idiom.
-    Meaning, if you write::
-
-      >>> with CmpH5Reader("aligned_reads.cmp.h5") as r:
-      ...   print r[0].read()
-
-    the `CmpH5Reader` object will be automatically closed after the
-    block within the "with" statement is executed.
-
-BAM/cmp.h5 compatibility: quick start
--------------------------------------
-
-If you have an application that uses the `CmpH5Reader` and you want to
-start using BAM files, your best bet is to use the following generic
-factory functions:
-
-.. autofunction:: pbcore.io.openIndexedAlignmentFile
-
-.. autofunction:: pbcore.io.openAlignmentFile
-
-.. note::
-
-   Since BAM files contain a subset of the information that was
-   present in cmp.h5 files, you will need to provide these functions
-   an indexed FASTA file for your reference.  For *full*
-   compatibility, you need the `openIndexedAlignmentFile` function,
-   which requires the existence of a `bam.pbi` file (PacBio BAM index
-   companion file).
-
-
-
-
-`bas.h5` / `bax.h5` Formats (PacBio basecalls file)
----------------------------------------------------
-
-The `bas.h5`/ `bax.h5` file formats are container formats for PacBio
-reads, built on top of the HDF5 standard.  Originally there was just
-one `bas.h5`, but eventually "multistreaming" came along and we had to
-split the file into three `bax.h5` *parts* and one `bas.h5` file
-containing pointers to the *parts*.  Use ``BasH5Reader`` to read any
-kind of `bas.h5` file, and ``BaxH5Reader`` to read a `bax.h5`.
-
-.. note::
-
-    In contrast to GFF, for example, the `bas.h5` read coordinate
-    system is 0-based and start-inclusive/end-exclusive, i.e. the same
-    convention as Python and the C++ STL.
-
-.. autoclass:: pbcore.io.BasH5Reader
-    :members:
-    :undoc-members:
-
-.. autoclass:: pbcore.io.BasH5IO.Zmw
-    :members:
-    :undoc-members:
-
-.. autoclass:: pbcore.io.BasH5IO.ZmwRead
-    :members:
-    :undoc-members:
-
-
 BAM format
 ----------
 
 The BAM format is a standard format described aligned and unaligned
-reads.  PacBio is transitioning from the cmp.h5 format to the BAM
-format.  For basic functionality, one should use :class:`BamReader`;
-for full compatibility with the :class:`CmpH5Reader` API (including
-alignment index functionality) one should use
-:class:`IndexedBamReader`, which requires the auxiliary *PacBio BAM
-index file* (``bam.pbi`` file).
+reads.  PacBio uses the BAM format exclusively.
+For basic functionality, one should use :class:`BamReader`;
+use :class:`IndexedBamReader` API for full index operation support,
+which requires the auxiliary *PacBio BAM index file* (``bam.pbi`` file).
 
 .. autoclass:: pbcore.io.BamAlignment
     :members:
@@ -126,29 +58,6 @@ index file* (``bam.pbi`` file).
     :undoc-members:
 
 
-
-`cmp.h5` format (legacy PacBio alignment file)
-----------------------------------------------
-
-The `cmp.h5` file format is an alignment format built on top of the HDF5
-standard.  It is a simple container format for PacBio alignment records.
-
-.. note::
-
-    In contrast to GFF, for example, all `cmp.h5` coordinate systems
-    (refererence, read) are 0-based and start-inclusive/end-exclusive,
-    i.e. the same convention as Python and the C++ STL.
-
-
-.. autoclass:: pbcore.io.CmpH5Reader
-    :members:
-    :undoc-members:
-
-.. autoclass:: pbcore.io.CmpH5Alignment
-    :members:
-    :undoc-members:
-
-
 FASTA Format
 ------------
 

pbcore/__init__.py

-from __future__ import absolute_import
+import pkg_resources
 
-# don't forget to update setup.py and doc/conf.py too
-__VERSION__ = "1.7.1"
+try:
+    __VERSION__ = pkg_resources.get_distribution('pbcore').version
+except Exception:
+    __VERSION__ = 'unknown'

pbcore/chemistry/__init__.py

-from __future__ import absolute_import
-
 from .chemistry import *

pbcore/chemistry/chemistry.py

-from __future__ import absolute_import
-
 __all__ = ["tripleFromMetadataXML",
            "decodeTriple",
            "ChemistryLookupError"]
 
-import xml.etree.ElementTree as ET, os.path
-from pkg_resources import Requirement, resource_filename
 from collections import OrderedDict
+import xml.etree.ElementTree as ET
+import os.path
+
+from pkg_resources import Requirement, resource_filename
+
+
+class ChemistryLookupError(Exception):
+    pass
 
-class ChemistryLookupError(Exception): pass
 
 def _loadBarcodeMappingsFromFile(mapFile):
     try:
@@ -16,30 +19,38 @@ def _loadBarcodeMappingsFromFile(mapFile):
         root = tree.getroot()
         mappingElements = root.findall("Mapping")
         mappings = OrderedDict()
-        mapKeys = ["BindingKit", "SequencingKit", "SoftwareVersion", "SequencingChemistry"]
+        mapKeys = ["BindingKit", "SequencingKit",
+                   "SoftwareVersion", "SequencingChemistry"]
         for mapElement in mappingElements:
             bindingKit = mapElement.find("BindingKit").text
             sequencingKit = mapElement.find("SequencingKit").text
             softwareVersion = mapElement.find("SoftwareVersion").text
             sequencingChemistry = mapElement.find("SequencingChemistry").text
-            mappings[(bindingKit, sequencingKit, softwareVersion)] = sequencingChemistry
+            mappings[(bindingKit, sequencingKit, softwareVersion)
+                     ] = sequencingChemistry
         return mappings
-    except:
+    except Exception:
         raise ChemistryLookupError("Error loading chemistry mapping xml")
 
+
 def _loadBarcodeMappings():
-    mappingFname = resource_filename(Requirement.parse('pbcore'),'pbcore/chemistry/resources/mapping.xml')
+    mappingFname = resource_filename(Requirement.parse(
+        'pbcore'), 'pbcore/chemistry/resources/mapping.xml')
     mappings = _loadBarcodeMappingsFromFile(mappingFname)
     updMappingDir = os.getenv("SMRT_CHEMISTRY_BUNDLE_DIR")
     if updMappingDir:
         import logging
         from os.path import join
-        logging.info("Loading updated chemistry mapping XML from {}".format(updMappingDir))
-        mappings.update(_loadBarcodeMappingsFromFile(join(updMappingDir, 'chemistry.xml')))
+        logging.info(
+            "Loading updated chemistry mapping XML from {}".format(updMappingDir))
+        mappings.update(_loadBarcodeMappingsFromFile(
+            join(updMappingDir, 'chemistry.xml')))
     return mappings
 
+
 _BARCODE_MAPPINGS = _loadBarcodeMappings()
 
+
 def tripleFromMetadataXML(metadataXmlPath):
     """
     Scrape the triple from the metadata.xml, or exception if the file
@@ -50,15 +61,20 @@ def tripleFromMetadataXML(metadataXmlPath):
     try:
         tree = ET.parse(metadataXmlPath)
         root = tree.getroot()
-        bindingKit = root.find("pb:BindingKit/pb:PartNumber", namespaces=nsd).text
-        sequencingKit = root.find("pb:SequencingKit/pb:PartNumber", namespaces=nsd).text
+        bindingKit = root.find(
+            "pb:BindingKit/pb:PartNumber", namespaces=nsd).text
+        sequencingKit = root.find(
+            "pb:SequencingKit/pb:PartNumber", namespaces=nsd).text
         # The instrument version is truncated to the first 2 dot delimited components
-        instrumentControlVersion = root.find("pb:InstCtrlVer", namespaces=nsd).text
+        instrumentControlVersion = root.find(
+            "pb:InstCtrlVer", namespaces=nsd).text
         verComponents = instrumentControlVersion.split(".")[0:2]
         instrumentControlVersion = ".".join(verComponents)
         return (bindingKit, sequencingKit, instrumentControlVersion)
     except Exception as e:
-        raise ChemistryLookupError("Could not find, or extract chemistry information from, %s" % (metadataXmlPath,))
+        raise ChemistryLookupError(
+            "Could not find, or extract chemistry information from, %s" % (metadataXmlPath,))
+
 
 def decodeTriple(bindingKit, sequencingKit, softwareVersion):
     """

pbcore/chemistry/resources/mapping.xml

 <?xml version="1.0" encoding="utf-8"?>
 <MappingTable>
-  <DefaultSequencingChemistry>XL-C2</DefaultSequencingChemistry>
-  <Mapping>
-    <SequencingChemistry>C2</SequencingChemistry>
-    <BindingKit>001672551</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>1.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>C2</SequencingChemistry>
-    <BindingKit>001672551</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>2.0</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>C2</SequencingChemistry>
-    <BindingKit>001672551</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>C2</SequencingChemistry>
-    <BindingKit>001672551</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>XL-C2</SequencingChemistry>
-    <BindingKit>100150800</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>1.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>XL-C2</SequencingChemistry>
-    <BindingKit>100150800</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>2.0</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>XL-C2</SequencingChemistry>
-    <BindingKit>100150800</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>XL-C2</SequencingChemistry>
-    <BindingKit>100150800</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>XL-XL</SequencingChemistry>
-    <BindingKit>100150800</BindingKit>
-    <SequencingKit>100180800</SequencingKit>
-    <SoftwareVersion>1.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>XL-XL</SequencingChemistry>
-    <BindingKit>100150800</BindingKit>
-    <SequencingKit>100180800</SequencingKit>
-    <SoftwareVersion>2.0</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>XL-XL</SequencingChemistry>
-    <BindingKit>100150800</BindingKit>
-    <SequencingKit>100180800</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>XL-XL</SequencingChemistry>
-    <BindingKit>100150800</BindingKit>
-    <SequencingKit>100180800</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P4-C2</SequencingChemistry>
-    <BindingKit>100236500</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>1.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P4-C2</SequencingChemistry>
-    <BindingKit>100236500</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>2.0</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P4-C2</SequencingChemistry>
-    <BindingKit>100236500</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P4-C2</SequencingChemistry>
-    <BindingKit>100236500</BindingKit>
-    <SequencingKit>001558034</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P4-XL</SequencingChemistry>
-    <BindingKit>100236500</BindingKit>
-    <SequencingKit>100180800</SequencingKit>
-    <SoftwareVersion>2.0</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P4-XL</SequencingChemistry>
-    <BindingKit>100236500</BindingKit>
-    <SequencingKit>100180800</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P4-XL</SequencingChemistry>
-    <BindingKit>100236500</BindingKit>
-    <SequencingKit>100180800</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P5-C3</SequencingChemistry>
-    <BindingKit>100256000</BindingKit>
-    <SequencingKit>100254800</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P5-C3</SequencingChemistry>
-    <BindingKit>100256000</BindingKit>
-    <SequencingKit>100254800</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P6-C4</SequencingChemistry>
-    <BindingKit>100356300</BindingKit>
-    <SequencingKit>100356200</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P6-C4</SequencingChemistry>
-    <BindingKit>100356300</BindingKit>
-    <SequencingKit>100356200</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P6-C4</SequencingChemistry>
-    <BindingKit>100372700</BindingKit>
-    <SequencingKit>100356200</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P6-C4</SequencingChemistry>
-    <BindingKit>100372700</BindingKit>
-    <SequencingKit>100356200</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P6-C4</SequencingChemistry>
-    <BindingKit>100356300</BindingKit>
-    <SequencingKit>100612400</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P6-C4</SequencingChemistry>
-    <BindingKit>100356300</BindingKit>
-    <SequencingKit>100612400</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P6-C4</SequencingChemistry>
-    <BindingKit>100372700</BindingKit>
-    <SequencingKit>100612400</SequencingKit>
-    <SoftwareVersion>2.1</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>P6-C4</SequencingChemistry>
-    <BindingKit>100372700</BindingKit>
-    <SequencingKit>100612400</SequencingKit>
-    <SoftwareVersion>2.3</SoftwareVersion>
-  </Mapping>
-
-  <!-- 3.0 ("Dromedary") chemistry -->
-  <Mapping>
-    <SequencingChemistry>S/P1-C1/beta</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-620-000</SequencingKit>
-    <SoftwareVersion>3.0</SoftwareVersion>
-  </Mapping>
-  <Mapping>
-    <SequencingChemistry>S/P1-C1/beta</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-620-000</SequencingKit>
-    <SoftwareVersion>3.1</SoftwareVersion>
-  </Mapping>
-
-  <!-- 3.1 ("Echidna") chemistry -->
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.1</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-867-300</SequencingKit>
-    <SoftwareVersion>3.1</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.1</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-867-300</SequencingKit>
-    <SoftwareVersion>3.2</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.1</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-867-300</SequencingKit>
-    <SoftwareVersion>3.3</SoftwareVersion>
-  </Mapping>
-
-  <!-- 3.1.1 ("Flea") chemistry -->
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.2</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-902-100</SequencingKit>
-    <SoftwareVersion>3.1</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.2</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-902-100</SequencingKit>
-    <SoftwareVersion>3.2</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.2</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-902-100</SequencingKit>
-    <SoftwareVersion>3.3</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.2</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-902-100</SequencingKit>
-    <SoftwareVersion>4.0</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.2</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-902-100</SequencingKit>
-    <SoftwareVersion>4.1</SoftwareVersion>
-  </Mapping>
-
-  <!---3.2 ("Goat") chemistry (a.k.a "Mimosa") -->
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.3</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-972-200</SequencingKit>
-    <SoftwareVersion>3.2</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.3</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-972-200</SequencingKit>
-    <SoftwareVersion>3.3</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.3</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-972-200</SequencingKit>
-    <SoftwareVersion>4.0</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P1-C1.3</SequencingChemistry>
-    <BindingKit>100-619-300</BindingKit>
-    <SequencingKit>100-972-200</SequencingKit>
-    <SoftwareVersion>4.1</SoftwareVersion>
-  </Mapping>
-
-  <!-- "Seabiscuit" chemistry, accompanied 4.0 SW -->
-  <Mapping>
-    <SequencingChemistry>S/P2-C2</SequencingChemistry>
-    <BindingKit>100-862-200</BindingKit>
-    <SequencingKit>100-861-800</SequencingKit>
-    <SoftwareVersion>4.0</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P2-C2</SequencingChemistry>
-    <BindingKit>100-862-200</BindingKit>
-    <SequencingKit>100-861-800</SequencingKit>
-    <SoftwareVersion>4.1</SoftwareVersion>
-  </Mapping>
-
-  <Mapping>
-    <SequencingChemistry>S/P2-C2</SequencingChemistry>
-    <BindingKit>100-862-200</BindingKit>
-    <SequencingKit>101-093-700</SequencingKit>
-    <SoftwareVersion>4.1</SoftwareVersion>
-  </Mapping>
+  <DefaultSequencingChemistry>S/P3-C1/5.0-8M</DefaultSequencingChemistry>
 
   <Mapping>
     <SequencingChemistry>S/P2-C2/5.0</SequencingChemistry>
@@ -449,4 +152,52 @@
     <SoftwareVersion>5.0</SoftwareVersion>
   </Mapping>
 
+  <!-- // Sequel® II Binding Kit 2.0; Sequel® II Sequencing Plate 2.0EA (4 Rxn) -->
+  <Mapping>
+    <SequencingChemistry>S/P4-C2/5.0-8M</SequencingChemistry>
+    <BindingKit>101-789-500</BindingKit>
+    <SequencingKit>101-789-300</SequencingKit>
+    <SoftwareVersion>5.0</SoftwareVersion>
+  </Mapping>
+
+  <!-- // Sequel® II Binding Kit 2.0; Sequel® II Sequencing Plate 2.0 (4 Rxn) -->
+  <Mapping>
+    <SequencingChemistry>S/P4-C2/5.0-8M</SequencingChemistry>
+    <BindingKit>101-789-500</BindingKit>
+    <SequencingKit>101-826-100</SequencingKit>
+    <SoftwareVersion>5.0</SoftwareVersion>
+  </Mapping>
+
+  <!-- // Sequel® II Binding Kit 2.0; Sequel® II Sequencing Plate 2.0 (4 Rxn) - QC -->
+  <Mapping>
+    <SequencingChemistry>S/P4-C2/5.0-8M</SequencingChemistry>
+    <BindingKit>101-789-500</BindingKit>
+    <SequencingKit>101-820-300</SequencingKit>
+    <SoftwareVersion>5.0</SoftwareVersion>
+  </Mapping>
+
+  <!-- // Sequel® II Binding Kit 2.1; Sequel® II Sequencing Plate 2.0EA (4 Rxn) -->
+  <Mapping>
+    <SequencingChemistry>S/P4.1-C2/5.0-8M</SequencingChemistry>
+    <BindingKit>101-820-500</BindingKit>
+    <SequencingKit>101-789-300</SequencingKit>
+    <SoftwareVersion>5.0</SoftwareVersion>
+  </Mapping>
+
+  <!-- // Sequel® II Binding Kit 2.1; Sequel® II Sequencing Plate 2.0 (4 Rxn) -->
+  <Mapping>
+    <SequencingChemistry>S/P4.1-C2/5.0-8M</SequencingChemistry>
+    <BindingKit>101-820-500</BindingKit>
+    <SequencingKit>101-826-100</SequencingKit>
+    <SoftwareVersion>5.0</SoftwareVersion>
+  </Mapping>
+
+  <!-- // Sequel® II Binding Kit 2.1; Sequel® II Sequencing Plate 2.0 (4 Rxn) - QC -->
+  <Mapping>
+    <SequencingChemistry>S/P4.1-C2/5.0-8M</SequencingChemistry>
+    <BindingKit>101-820-500</BindingKit>
+    <SequencingKit>101-820-300</SequencingKit>
+    <SoftwareVersion>5.0</SoftwareVersion>
+  </Mapping>
+
 </MappingTable>