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racon (1.3.1-1) UNRELEASED; urgency=medium
racon (1.3.1-1) unstable; urgency=medium
* Initial release (Closes: #890187)
TODO:
https://salsa.debian.org/med-team/libbioparser-dev (#901064)
https://salsa.debian.org/med-team/libedlib-dev (#901184)
https://salsa.debian.org/med-team/rampler (waiting for proper description)
https://salsa.debian.org/med-team/spoa (#901145)
https://salsa.debian.org/med-team/libthread-pool (#901207)
-- Andreas Tille <tille@debian.org> Fri, 08 Jun 2018 13:20:51 +0200
-- Andreas Tille <tille@debian.org> Sun, 10 Jun 2018 11:21:59 +0200
Source: racon
Section: science
Priority: optional
Maintainer: Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.org>
Uploaders: Cédric Lood <cedric.lood@kuleuven.be>,
Andreas Tille <tille@debian.org>
Section: science
Priority: optional
Build-Depends: debhelper (>= 11~),
cmake,
libgtest-dev,
......@@ -19,13 +19,36 @@ Homepage: https://github.com/isovic/racon
Package: racon
Architecture: any
Depends: ${shlibs:Depends}, ${misc:Depends}
Depends: ${shlibs:Depends},
${misc:Depends}
Description: consensus module for raw de novo DNA assembly of long uncorrected reads
Racon is intended as a standalone consensus module to correct raw
contigs generated by rapid assembly methods which do not include a
consensus step, such as Miniasm.
consensus step. The goal of Racon is to generate genomic consensus which
is of similar or better quality compared to the output generated by
assembly methods which employ both error correction and consensus steps,
while providing a speedup of several times compared to those methods. It
supports data produced by both Pacific Biosciences and Oxford Nanopore
Technologies.
.
Racon can be used as a polishing tool after the assembly with either
Illumina data or data produced by third generation of sequencing. The
type of data inputed is automatically detected.
.
Racon takes as input only three files: contigs in FASTA/FASTQ format,
reads in FASTA/FASTQ format and overlaps/alignments between the reads
and the contigs in MHAP/PAF/SAM format. Output is a set of polished
contigs in FASTA format printed to stdout. All input files can be
compressed with gzip.
.
Racon can also be used as a read error-correction tool. In this
scenario, the MHAP/PAF/SAM file needs to contain pairwise overlaps
between reads including dual overlaps.
.
The goal of Racon is to generate genomic consensus which is of similar
or better quality compared to the output generated by assembly methods
which employ both error correction and consensus steps, while
providing a speedup of several times compared to those m
A wrapper script is also available to enable easier usage to the end-
user for large datasets. It has the same interface as racon but adds
two additional features from the outside. Sequences can be subsampled
to decrease the total execution time (accuracy might be lower) while
target sequences can be split into smaller chunks and run sequentially
to decrease memory consumption. Both features can be run at the same
time as well.
#!/bin/sh
MANDIR=debian
mkdir -p $MANDIR
VERSION=`dpkg-parsechangelog | awk '/^Version:/ {print $2}' | sed -e 's/^[0-9]*://' -e 's/-.*//' -e 's/[+~]dfsg$//'`
AUTHOR=".SH AUTHOR\nThis manpage was written by $DEBFULLNAME for the Debian distribution and
can be used for any other usage of the program.
"
progname=racon
help2man --no-info --no-discard-stderr --help-option=" --help" \
--name='consensus module for raw de novo DNA assembly of long uncorrected reads' \
--version-string="$VERSION" ${progname} > $MANDIR/${progname}.1
echo $AUTHOR >> $MANDIR/${progname}.1
cat <<EOT
Please enhance the help2man output.
The following web page might be helpful in doing so:
http://liw.fi/manpages/
EOT
.TH RACON "1" "June 2018" "racon 1.3.1" "User Commands"
.SH NAME
racon \- consensus module for raw de novo DNA assembly of long uncorrected reads
.SH SYNPOPSIS
.B racon
[options ...] <sequences> <overlaps> <target sequences>
.SH DESCRIPTION
Racon is intended as a standalone consensus module to correct raw
contigs generated by rapid assembly methods which do not include a
consensus step. The goal of Racon is to generate genomic consensus which
is of similar or better quality compared to the output generated by
assembly methods which employ both error correction and consensus steps,
while providing a speedup of several times compared to those methods. It
supports data produced by both Pacific Biosciences and Oxford Nanopore
Technologies.
.P
Racon can be used as a polishing tool after the assembly with either
Illumina data or data produced by third generation of sequencing. The
type of data inputed is automatically detected.
.P
Racon takes as input only three files: contigs in FASTA/FASTQ format,
reads in FASTA/FASTQ format and overlaps/alignments between the reads
and the contigs in MHAP/PAF/SAM format. Output is a set of polished
contigs in FASTA format printed to stdout. All input files can be
compressed with gzip.
.P
Racon can also be used as a read error-correction tool. In this
scenario, the MHAP/PAF/SAM file needs to contain pairwise overlaps
between reads including dual overlaps.
.P
A wrapper script is also available to enable easier usage to the end-
user for large datasets. It has the same interface as racon but adds
two additional features from the outside. Sequences can be subsampled
to decrease the total execution time (accuracy might be lower) while
target sequences can be split into smaller chunks and run sequentially
to decrease memory consumption. Both features can be run at the same
time as well.
.SH OPTIONS
.HP
<sequences>
.IP
input file in FASTA/FASTQ format (can be compressed with gzip)
containing sequences used for correction
.HP
<overlaps>
.IP
input file in MHAP/PAF/SAM format (can be compressed with gzip)
containing overlaps between sequences and target sequences
.HP
<target sequences>
.IP
input file in FASTA/FASTQ format (can be compressed with gzip)
containing sequences which will be corrected
.SS optional
.HP
\fB\-u\fR, \fB\-\-include\-unpolished\fR
.IP
output unpolished target sequences
.HP
\fB\-f\fR, \fB\-\-fragment\-correction\fR
.IP
perform fragment correction instead of contig polishing
(overlaps file should contain dual/self overlaps!)
.HP
\fB\-w\fR, \fB\-\-window\-length\fR <int>
.IP
default: 500
size of window on which POA is performed
.HP
\fB\-q\fR, \fB\-\-quality\-threshold\fR <float>
.IP
default: 10.0
threshold for average base quality of windows used in POA
.HP
\fB\-e\fR, \fB\-\-error\-threshold\fR <float>
.IP
default: 0.3
maximum allowed error rate used for filtering overlaps
.HP
\fB\-m\fR, \fB\-\-match\fR <int>
.IP
default: 5
score for matching bases
.HP
\fB\-x\fR, \fB\-\-mismatch\fR <int>
.IP
default: \fB\-4\fR
score for mismatching bases
.HP
\fB\-g\fR, \fB\-\-gap\fR <int>
.IP
default: \fB\-8\fR
gap penalty (must be negative)
.HP
\fB\-t\fR, \fB\-\-threads\fR <int>
.IP
default: 1
number of threads
.HP
\fB\-\-version\fR
.IP
prints the version number
.HP
\fB\-h\fR, \fB\-\-help\fR
.IP
prints the usage
.SH AUTHOR
This manpage was written by Andreas Tille for the Debian distribution and can be used for any other usage of the program.