Commit b12160d8 authored by Afif Elghraoui's avatar Afif Elghraoui
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New upstream version 2.0.0

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Archaea-BHB-noncan.cm

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INFERNAL-1 [1.0]
NAME     Archaea-NC-Intron
STATES   124
NODES    28
ALPHABET 1
ELSELF   -0.08926734
WBETA    1e-07
NSEQ     148
EFFNSEQ  148.000
CLEN     40
BCOM     cmbuild --rf --enone -F Archaea-BHB-noncan.cm Archaea-BHB-noncan.sto
BDATE    Wed Aug  5 18:38:10 2009
NULL     0.000  0.000  0.000  0.000 
MODEL:
				[ ROOT    0 ]
     S     0    -1 0     1     6 -13.180 -13.120  -0.001 -11.896 -12.176 -12.571 
    IL     1     1 2     1     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR     2     2 3     2     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP    1 ]
    MP     3     2 3     7     6 -13.180 -13.120  -0.001 -11.896 -12.176 -12.571 -0.553  0.180 -0.805 -1.580  0.039 -0.132  1.032 -0.961  1.087  0.350 -0.302 -3.122 -0.376  0.222  0.847 -0.473 
    ML     4     2 3     7     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR     5     2 3     7     6  -6.988  -5.717  -1.625  -5.695  -0.829  -3.908  0.660 -0.612 -0.293 -0.076 
     D     6     2 3     7     6  -9.049  -7.747  -3.544  -4.226  -4.244  -0.319 
    IL     7     7 5     7     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR     8     8 6     8     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP    2 ]
    MP     9     8 6    13     6 -13.180 -13.120  -0.001 -11.896 -12.176 -12.571 -0.679  0.345 -1.278  0.217 -1.335  0.610  0.158 -2.094 -0.942  1.328  0.852 -0.808 -0.468  0.384  0.241 -0.638 
    ML    10     8 6    13     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR    11     8 6    13     6  -6.988  -5.717  -1.625  -5.695  -0.829  -3.908  0.660 -0.612 -0.293 -0.076 
     D    12     8 6    13     6  -9.049  -7.747  -3.544  -4.226  -4.244  -0.319 
    IL    13    13 5    13     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    14    14 6    14     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP    3 ]
    MP    15    14 6    19     6 -13.180 -13.120  -0.001 -11.896 -12.176 -12.571 -3.085 -0.094  0.388  0.575 -4.100 -1.284  0.323 -0.315  0.485  1.652  0.861  0.534 -1.310 -0.940 -0.533 -2.306 
    ML    16    14 6    19     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR    17    14 6    19     6  -6.988  -5.717  -1.625  -5.695  -0.829  -3.908  0.660 -0.612 -0.293 -0.076 
     D    18    14 6    19     6  -9.049  -7.747  -3.544  -4.226  -4.244  -0.319 
    IL    19    19 5    19     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    20    20 6    20     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP    4 ]
    MP    21    20 6    25     6 -13.180 -13.120  -0.001 -11.896 -12.176 -12.571 -1.627  1.032  0.144  0.574 -0.502  0.326  0.474 -1.619 -0.731  1.846 -0.268 -0.507 -2.731 -2.115  0.115 -2.573 
    ML    22    20 6    25     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR    23    20 6    25     6  -6.988  -5.717  -1.625  -5.695  -0.829  -3.908  0.660 -0.612 -0.293 -0.076 
     D    24    20 6    25     6  -9.049  -7.747  -3.544  -4.226  -4.244  -0.319 
    IL    25    25 5    25     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    26    26 6    26     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP    5 ]
    MP    27    26 6    31     4 -12.154  -6.639  -0.016  -9.853                 -4.149 -2.051  0.059 -2.692 -0.481 -0.348  1.371 -0.318 -1.425  2.266 -0.515  0.197 -1.021 -0.781 -0.408 -0.402 
    ML    28    26 6    31     4  -2.408  -4.532  -1.293  -1.473                  0.660 -0.612 -0.293 -0.076 
    MR    29    26 6    31     4  -4.102 -12.528  -0.390  -2.485                  0.660 -0.612 -0.293 -0.076 
     D    30    26 6    31     4 -12.737 -14.007  -2.036  -0.404                 
    IL    31    31 5    31     4  -2.817  -4.319  -0.613  -2.698                  0.000  0.000  0.000  0.000 
    IR    32    32 6    32     3  -3.062  -0.226  -5.301                          0.000  0.000  0.000  0.000 
				[ MATR    6 ]
    MR    33    32 6    35     3 -13.564  -0.001 -11.882                         -0.041 -0.728  0.409  0.133 
     D    34    32 6    35     3  -6.390  -1.568  -0.620                         
    IR    35    35 3    35     3  -1.925  -0.554  -4.164                          0.000  0.000  0.000  0.000 
				[ MATR    7 ]
    MR    36    35 3    38     3 -13.564  -0.001 -11.882                          0.773 -1.034  0.652 -2.111 
     D    37    35 3    38     3  -6.390  -1.568  -0.620                         
    IR    38    38 3    38     3  -1.925  -0.554  -4.164                          0.000  0.000  0.000  0.000 
				[ MATR    8 ]
    MR    39    38 3    41     5 -12.207  -0.001 -12.022 -12.234 -13.126          1.907 -3.509 -3.041 -4.637 
     D    40    38 3    41     5  -5.352  -0.707  -2.978  -4.409  -2.404         
    IR    41    41 3    41     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP    9 ]
    MP    42    41 3    46     6 -13.180 -13.120  -0.001 -11.896 -12.176 -12.571 -4.303 -2.311 -0.025 -0.706 -4.219 -1.631  2.474 -4.243 -2.815  1.438 -4.589  1.376  0.264 -1.749  0.162 -4.067 
    ML    43    41 3    46     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR    44    41 3    46     6  -6.988  -5.717  -1.625  -5.695  -0.829  -3.908  0.660 -0.612 -0.293 -0.076 
     D    45    41 3    46     6  -9.049  -7.747  -3.544  -4.226  -4.244  -0.319 
    IL    46    46 5    46     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    47    47 6    47     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP   10 ]
    MP    48    47 6    52     6 -13.180 -13.120  -0.001 -11.896 -12.176 -12.571 -6.894 -3.183 -7.771  0.944 -8.050 -7.689  2.211 -7.339 -7.035  2.653 -2.529 -1.595  0.838 -7.036 -0.516 -6.190 
    ML    49    47 6    52     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR    50    47 6    52     6  -6.988  -5.717  -1.625  -5.695  -0.829  -3.908  0.660 -0.612 -0.293 -0.076 
     D    51    47 6    52     6  -9.049  -7.747  -3.544  -4.226  -4.244  -0.319 
    IL    52    52 5    52     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    53    53 6    53     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP   11 ]
    MP    54    53 6    58     6 -13.180 -13.120  -0.001 -11.896 -12.176 -12.571 -7.157 -6.147 -8.101  0.723 -8.763 -7.957  1.793 -7.599 -7.277  3.124 -6.961 -0.665 -0.023 -7.318 -1.411 -3.113 
    ML    55    53 6    58     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR    56    53 6    58     6  -6.988  -5.717  -1.625  -5.695  -0.829  -3.908  0.660 -0.612 -0.293 -0.076 
     D    57    53 6    58     6  -9.049  -7.747  -3.544  -4.226  -4.244  -0.319 
    IL    58    58 5    58     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    59    59 6    59     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP   12 ]
    MP    60    59 6    64     4 -12.357 -12.564  -0.001 -10.978                 -6.403 -7.081 -0.862 -0.921 -6.146 -2.492  2.202 -7.461 -1.224  2.604 -7.540 -5.329 -1.275 -6.909  1.647 -6.316 
    ML    61    59 6    64     4  -3.758  -3.940  -0.507  -2.670                  0.660 -0.612 -0.293 -0.076 
    MR    62    59 6    64     4  -4.809  -3.838  -1.706  -0.766                  0.660 -0.612 -0.293 -0.076 
     D    63    59 6    64     4  -4.568  -4.250  -2.265  -0.520                 
    IL    64    64 5    64     4  -1.686  -2.369  -1.117  -4.855                  0.000  0.000  0.000  0.000 
    IR    65    65 6    65     3  -1.442  -0.798  -4.142                          0.000  0.000  0.000  0.000 
				[ MATL   13 ]
    ML    66    65 6    68     3 -13.269  -0.001 -11.923                          0.690 -0.282  0.156 -1.154 
     D    67    65 6    68     3  -6.174  -1.687  -0.566                         
    IL    68    68 3    68     3  -1.442  -0.798  -4.142                          0.000  0.000  0.000  0.000 
				[ MATL   14 ]
    ML    69    68 3    71     3 -13.269  -0.001 -11.923                         -0.439  0.123  0.805 -1.229 
     D    70    68 3    71     3  -6.174  -1.687  -0.566                         
    IL    71    71 3    71     3  -1.442  -0.798  -4.142                          0.000  0.000  0.000  0.000 
				[ MATL   15 ]
    ML    72    71 3    74     5 -12.207  -0.047  -6.886  -7.136  -5.962          1.545 -2.377 -1.607 -0.833 
     D    73    71 3    74     5  -4.959  -0.803  -4.221  -2.596  -2.508         
    IL    74    74 3    74     5  -2.408  -0.496  -4.087  -5.920  -5.193          0.000  0.000  0.000  0.000 
				[ MATP   16 ]
    MP    75    74 3    79     6 -13.136 -13.076  -0.060 -11.852  -5.374  -5.957 -11.758 -10.096 -11.268 -2.214 -8.937 -12.516  2.537 -10.139 -12.707  2.974 -11.780 -1.382  0.621 -12.752 -2.356 -8.766 
    ML    76    74 3    79     6  -7.725  -8.071  -2.786  -2.480  -7.921  -0.592  1.593 -1.856 -1.723 -1.306 
    MR    77    74 3    79     6  -8.189  -6.918  -2.827  -6.897  -0.304  -5.110  1.532 -1.693 -1.530 -1.142 
     D    78    74 3    79     6 -11.481 -10.179  -5.976  -6.659  -6.676  -0.054 
    IL    79    79 5    79     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    80    80 6    80     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP   17 ]
    MP    81    80 6    85     6 -13.078 -13.017  -0.176 -11.793  -5.650  -3.410 -6.750 -5.892 -2.749  0.736 -1.367 -7.583  1.586 -7.223 -3.149  3.082 -6.660 -1.920  0.457 -6.909 -0.988 -6.076 
    ML    82    80 6    85     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR    83    80 6    85     6  -9.755  -8.484  -0.495  -8.462  -1.869  -6.675 -0.920 -1.582  0.789  0.496 
     D    84    80 6    85     6 -12.642 -11.340  -7.137  -7.819  -7.837  -0.024 
    IL    85    85 5    85     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    86    86 6    86     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP   18 ]
    MP    87    86 6    91     6 -12.944 -12.883  -0.328 -11.659  -6.872  -2.366 -3.574 -3.158 -2.975  1.051 -7.669 -7.356  2.093 -2.601 -2.084  2.718 -6.456 -0.740  0.602 -6.710 -2.354 -5.916 
    ML    88    86 6    91     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR    89    86 6    91     6  -9.540  -8.269  -0.177  -8.247  -3.381  -6.460  0.375  0.213 -0.189 -0.586 
     D    90    86 6    91     6 -14.258 -12.956  -8.753  -9.436  -9.453  -0.008 
    IL    91    91 5    91     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    92    92 6    92     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP   19 ]
    MP    93    92 6    97     6 -12.670 -12.610  -0.354 -11.386  -6.521  -2.281 -6.550 -2.506 -7.481  0.718 -8.079 -7.352  2.827 -2.550 -6.675  2.376 -6.366 -0.382 -0.569 -6.707 -2.291 -5.871 
    ML    94    92 6    97     6  -6.250  -6.596  -1.310  -1.005  -6.446  -3.975  0.660 -0.612 -0.293 -0.076 
    MR    95    92 6    97     6  -8.230  -6.959  -2.868  -6.937  -0.295  -5.151  1.550 -1.738 -1.583 -1.187 
     D    96    92 6    97     6 -15.439 -14.137  -9.934 -10.616 -10.634  -0.003 
    IL    97    97 5    97     6  -2.579  -2.842  -0.760  -4.497  -5.274  -4.934  0.000  0.000  0.000  0.000 
    IR    98    98 6    98     5  -2.408  -0.496  -5.920  -4.087  -5.193          0.000  0.000  0.000  0.000 
				[ MATP   20 ]
    MP    99    98 6   103     4 -11.450 -11.657  -0.895  -1.115                 -5.905 -5.479 -2.092  0.063 -5.605 -6.755  2.924 -6.315 -2.962  2.213 -6.075 -0.726  0.130 -6.037 -0.914 -5.097 
    ML   100    98 6   103     4  -3.758  -3.940  -0.507  -2.670                  0.660 -0.612 -0.293 -0.076 
    MR   101    98 6   103     4  -6.893  -5.922  -0.258  -2.850                  0.633 -1.416  0.599 -0.836 
     D   102    98 6   103     4 -11.437 -11.119  -9.134  -0.004                 
    IL   103   103 5   103     4  -1.686  -2.369  -1.117  -4.855                  0.000  0.000  0.000  0.000 
    IR   104   104 6   104     3  -1.442  -0.798  -4.142                          0.000  0.000  0.000  0.000 
				[ MATL   21 ]
    ML   105   104 6   107     3 -11.550  -0.002 -10.204                         -1.502  0.882  0.329 -0.868 
     D   106   104 6   107     3 -14.171  -0.004  -8.562                         
    IL   107   107 3   107     3  -1.442  -0.798  -4.142                          0.000  0.000  0.000  0.000 
				[ MATL   22 ]
    ML   108   107 3   110     3 -13.269  -0.011  -7.094                          0.051  0.101  0.211 -0.445 
     D   109   107 3   110     3  -6.174  -1.687  -0.566                         
    IL   110   110 3   110     3  -1.442  -0.798  -4.142                          0.000  0.000  0.000  0.000 
				[ MATL   23 ]
    ML   111   110 3   113     3  -0.900  -2.036  -2.183                         -0.109  0.249  0.323 -0.660 
     D   112   110 3   113     3  -8.015  -0.308  -2.406                         
    IL   113   113 3   113     3  -0.249  -2.656 -12.425                          0.000  0.000  0.000  0.000 
				[ MATL   24 ]
    ML   114   113 3   116     3 -12.913  -0.001 -11.567                          0.106 -0.048 -0.126  0.056 
     D   115   113 3   116     3 -12.510  -0.012  -6.902                         
    IL   116   116 3   116     3  -1.442  -0.798  -4.142                          0.000  0.000  0.000  0.000 
				[ MATL   25 ]
    ML   117   116 3   119     3 -13.269  -1.720  -0.522                          0.226 -0.054  0.162 -0.417 
     D   118   116 3   119     3  -6.174  -1.687  -0.566                         
    IL   119   119 3   119     3  -1.442  -0.798  -4.142                          0.000  0.000  0.000  0.000 
				[ MATL   26 ]
    ML   120   119 3   122     2       *   0.000                                 -1.563  0.297  0.962 -1.042 
     D   121   119 3   122     2       *   0.000                                 
    IL   122   122 3   122     2  -1.823  -0.479                                  0.000  0.000  0.000  0.000 
				[ END    27 ]
     E   123   122 3    -1     0                                                 
//
+4 −4
Original line number Diff line number Diff line
tRNAscan-SE -- a program for finding transfer RNAs

Copyright (C) 1996 Todd M.J. Lowe & Sean R. Eddy
Copyright (C) 2017 Patricia P. Chan, Brian Lin, and Todd M. Lowe

This set of programs is free software; you can redistribute it and/or
modify it under the terms of the GNU General Public License as
published by the Free Software Foundation; either version 2 of the
published by the Free Software Foundation; either version 3 of the
License, or (at your option) any later version.

This program is distributed in the hope that it will be useful, but
@@ -13,8 +13,8 @@ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
General Public License for more details.

You should have received a copy of the GNU General Public License
along with this program, in the file GNULICENSE; if not, write to the Free
Software Foundation, Inc., 675 Mass. Ave, Cambridge, MA 02139 USA.
along with this program, in the file GNULICENSE; if not, check out
the web site at https://www.gnu.org/licenses/gpl-3.0.en.html.

This distribution includes source code originally derived from other
sources.  In particular, modified source code from the program

Cove/COPYING

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cove -- programs to apply covariance models to RNA sequence analysis

Copyright (C) 1993,1994 Sean R. Eddy

This set of programs is free software; you can redistribute it and/or
modify it under the terms of the GNU General Public License as
published by the Free Software Foundation; either version 2 of the
License, or (at your option) any later version.

This program is distributed in the hope that it will be useful, but
WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
General Public License for more details.

You should have received a copy of the GNU General Public License
along with this program, in the file GNULICENSE; if not, write to the Free
Software Foundation, Inc., 675 Mass. Ave, Cambridge, MA 02139 USA.

This distribution may include source code originally derived from
other sources.  In particular, my general sequence library includes
source code that derives from Henry Spencer (regexp() functions), and
Don Gilbert (sequence i/o functions).  Borrowed code is annotated and
credited in the source files whenever it appears. The copying policies
of my benefactors are compatible with the GNU Public License under
which this package is distributed.


Cove/DEMO

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Demonstrations of the covariance model programs
-----------------------------------------------

As an introduction to how the software works, this file contains
instructions on how to reproduce the results in our paper.  (Note
that this version of the code is newer, somewhat less buggy, and
faster than the version used for our original experiments. There may
be minor differences between the results we report in our paper and
the results you get from these demos.)

For more details, refer to S.R. Eddy and R. Durbin, "RNA Sequence
Analysis Using Covariance Models", NAR 22:2079-2088, 1994.

The directory Demos/ contains the data files:

   TRNA2.cm        - A very good tRNA model
   7SL-euk.cm      - A model of 7SL signal recognition particle RNA
   7SL-euk.fa      - 37 eukaryotic 7SL RNAs (includes some fragments)	
   trna1415.slx    - Steinberg/Sprinzl trusted tRNA alignment
   sim65.slx       - alignment of 100 dissimilar tRNAs
   sim100.slx      - alignment of 100 randomly chosen tRNAs
   test100.fa      - 100 unaligned, randomly chosen independent test tRNAs
   sim65.fa        - unaligned SIM65 sequences
   sim100.fa       - unaligned SIM100 sequences
   fig5.slx        - Trusted alignment of 5 tRNAs, from our figure 5
   fig5.fa         - unaligned tRNA sequences of fig5.slx
   yeast-phe.fa    - S. cerevisiae tRNA-Phe sequence	
   K11H3frag.fa    - fragment of C. elegans cosmid K11H3, which contains 2 tRNA genes

## Constructing a model from trusted alignments:

> covet -a sim100.slx A100.cm sim100.fa
> covet -a sim65.slx A65.cm sim65.fa
     
  Produces models A100 and A65. Uses trusted alignments (sim100.slx,
  sim65.slx) to construct the initial models; then uses sim100.fa or
  sim65.fa as training sequences.
  Takes about fifteen minutes per model on an R4000 Indigo.
 
## Constructing a model from unaligned sequences:

> covet U100.cm sim100.fa
> covet U65.cm sim65.fa

  Produces models U100 and U65 from unaligned training sequences in sim100.fa
  and sim65.fa.
  Takes about an hour per model on an R4000 Indigo.

## Multiple RNA sequence alignment using a trained model:

> covea A100.cm fig5.fa
> covea U100.cm fig5.fa

  Produces a multiple sequence alignment of the 5 sequences in fig5.fa
  and prints it to the screen. Compare to the trusted alignment
  (fig5.slx) or our preprint's Figure 5.
  Takes about ten seconds.
   

## Scoring example sequences using a trained model:

> coves A100.cm fig5.fa

  Calculates and prints alignment scores (in bits) for the 5 sequences in
  fig5.fa.
  Takes about ten seconds.

## Structure prediction of individual sequences using a trained model:
 
> coves -s A100.cm yeast-phe.fa
> coves -m A100.cm yeast-phe.fa

  Aligns the model A100.cm to yeast tRNA-Phe, and prints out the alignment
  score. The -s option to coves prints out a secondary structure representation
  for the sequence as well. This representation uses ">" and "<" characters
  to represent pairwise assignments. The -m option to coves produces a
  "mountain" representation of the structure. Both of these representations
  are described in a paper by Danielle Konings (Konings and Hogeweg,
  J. Mol. Biol. 207:597-614, 1989).  Covariance models typically
  overpredict pairs; the predicted pairs are a superset of the
  secondary structure pairings. [One of the major weaknesses in the package at the 
  moment is a lack of graphical secondary structure display and a lack of 
  automatic classification of predicted pairs as secondary structure vs. tertiary 
  structure or overprediction.]
  Takes about ten seconds.	
	
## Searching a database for new tRNAs:

> covels -c A100.cm K11H3frag.fa

  Searches a 500 bp fragment of the C. elegans cosmid K11H3 for tRNAs,
  on both strands (the -c option asks for both strands to be searched).
  This fragment contains two predicted tRNA genes.
  Takes about two minutes.

> covels -c -w 150 TRNA2.cm K11H3frag.fa

  Same as above, except using a model trained on 1315 different tRNA
  sequences. This model is known to recognize all known cytoplasmic 
  eukaryotic tRNAs with scores > 20 bits. The -w option allows tRNAs
  up to 150 nt in length. Using covels with this model is the most 
  sensitive means I know of for detecting tRNAs in genomic sequence. 

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